3D細(xì)胞培養(yǎng)，如球狀體、類器官或3D支架中的細(xì)胞，越來越多地用于體外研究，與傳統(tǒng)的2D培養(yǎng)相比，它提供了更精確的體內(nèi)環(huán)境表征。而現(xiàn)在，隨著先進(jìn)的培養(yǎng)方法，如動(dòng)態(tài)流動(dòng)培養(yǎng)或生物打印，可以創(chuàng)建更接近生物起源的3D組織條件。因此，3D細(xì)胞培養(yǎng)模型和先進(jìn)的培養(yǎng)方法在細(xì)胞活力、基因表達(dá)、疾病建模和藥物測試以及毒性研究方面帶來了更多生理學(xué)相關(guān)的結(jié)果。
ibidi為3D細(xì)胞模型的創(chuàng)建和基于圖像的分析提供了先進(jìn)的、可定制的解決方案。ibidi的模塊化系統(tǒng)旨在簡化尖端3D細(xì)胞分析的復(fù)雜性。通過整合ibidi產(chǎn)品的各種組件，研究人員可以毫不費(fèi)力地管理復(fù)雜的設(shè)計(jì)，提高其科學(xué)研究的穩(wěn)健性和效率。使用ibidi µ-Slides/µ-Dishes/µ-Plates、ibidi Pump System或ibidi Stage Top Incubators，可以將您的研究提升到一個(gè)新的水平，并獲得更準(zhǔn)確可靠的結(jié)果。
實(shí)驗(yàn)工作流程
從樣品制備到3D細(xì)胞培養(yǎng)、成像和分析
系統(tǒng)和應(yīng)用
3D細(xì)胞培養(yǎng)實(shí)驗(yàn)需要具有絕對(duì)精度和可靠性的穩(wěn)健設(shè)置。ibidi產(chǎn)品，包括µ-Slides/ µ-Dishes/ µ-Plates、ibidi Pump System和 ibidi Stage Top Incubators，專為在生理?xiàng)l件下進(jìn)行和分析3D細(xì)胞培養(yǎng)試驗(yàn)而設(shè)計(jì)。由于其靈活性和模塊化，它們可以很容易地適應(yīng)您的實(shí)驗(yàn)需求。
用于3D細(xì)胞模型分析的ibidi解決方案
參考文獻(xiàn)
1、Human blastoids were cultured on a 3D matrix using the µ-Slide 8 Well.
Karvas RM, Zemke JE, Ali SS, Upton E, Sane E, Fischer LA, Dong C, Park KM, Wang F, Park K, Hao S, Chew B, Meyer B, Zhou C, Dietmann S, Theunissen TW. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages. Cell Stem Cell., 2023, 10.1016/j.stem.2023.08.005.
2、3D sandwich cultures of squameous cell carcinoma cells were done in the µ-Plate 96 Well 3D.
E. Hoque Apu, S.U. Akram, J. Rissanen, H. Wan and T. Salo. Desmoglein 3 – Influence on oral carcinoma cell migration and invasion. Experimental Cell Research, 2018, 10.1016/j.yexcr.2018.06.037.
3、The 12 Well Chamber, removable was used for the cultivation of 3D human acinar ductal metaplasia cells.
da Silva, L., Jiang, J., Perkins, C. et al. Pharmacological inhibition and reversal of pancreatic acinar ductal metaplasia. Cell Death Discov., 2022, 10.1038/s41420-022-01165-4.
4、3D cell culture of human breast epithelial MCF10A cells was done in the µ-Slide 8 Well.
H. Grobe, A. Wüstenhagen, C. Baarlink, R. Grosse and K. Grikscheit. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PloS one, 2018, 10.1371/journal.pone.0194716.
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